Current Events > Ask me nearly anything

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PiOverlord 03/27/20 1:28:27 PM #1:	I feel like CE and me just aren't a thing anymore. --- Number of legendary 500 post topics: 33, 500th posts: 28; PiO ATTN: 5 RotM wins 1, LETTEN MY ARROW FLYEN TRUE <cite>PiOverlord posted [p:936323360] in Ask me nea... [t:78512185]...</cite> <quote>I feel like CE and me just aren't a thing anymore. </quote> ... Copied to Clipboard!
Kazi1212 03/27/20 1:29:12 PM #2:	Are you part of the Fam? --- My mind is an open book, do you find it entertaining? At least be original in your responses then. "Don't call me a God, call me a Sadist" <cite>Kazi1212 posted [p:936323403] in Ask me nea... [t:78512185]...</cite> <quote>Are you part of the Fam? </quote> ... Copied to Clipboard!
MedeaLysistrata 03/27/20 1:29:25 PM #3:	Fave JRPG? --- "Why is ontology so expensive?" - JH [Is this live?][Joyless planet...] <cite>MedeaLysistrata posted [p:936323415] in Ask me nea... [t:78512185]...</cite> <quote>Fave JRPG? </quote> ... Copied to Clipboard!
Lost_All_Senses 03/27/20 1:29:33 PM #4:	Why don't you look at me the way you use to? --- Name checks out "Stupidity is a spectrum, and we're all on it" - OfDustandBone <cite>Lost_All_Senses posted [p:936323423] in Ask me nea... [t:78512185]...</cite> <quote>Why don't you look at me the way you use to? </quote> ... Copied to Clipboard!
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AbraCadaver 03/27/20 1:30:28 PM #6:	What is the air speed velocity of an unladen swallow? --- Yo ho ho and a bottle of...dumb. <cite>AbraCadaver posted [p:936323469] in Ask me nea... [t:78512185]...</cite> <quote>What is the air speed velocity of an unladen swallow? </quote> ... Copied to Clipboard!
PiOverlord 03/27/20 1:42:22 PM #7:	Kazi1212 posted... Are you part of the Fam? Yeah. I know a few take issue with that. MedeaLysistrata posted... Fave JRPG? Great question! God, that's tough. I've played only a few but loved them all. Octopath was really fun and sticks out to me. Valkyria Chronicles too. Lost_All_Senses posted... Why don't you look at me the way you use to? I feel like all my CEmmunity building attempts has just not worked. Just feels like I'm chasing a lost thing that'll never return. [LFAQs-redacted-quote] Super Mario Odyssey, Breath of the Wild, and Super Mario Party. Been speedrunning SMO for a while, it's my favorite game ever, but I recently learned the first half of BotW any%, which has been fun. AbraCadaver posted... What is the air speed velocity of an unladen swallow? Will this be on the test? --- Number of legendary 500 post topics: 33, 500th posts: 28; PiO ATTN: 5 RotM wins 1, LETTEN MY ARROW FLYEN TRUE <cite>PiOverlord posted [p:936324100] in Ask me nea... [t:78512185]...</cite> <quote>Kazi1212 posted... </cite> <quote>Are you part of the Fam?</quote>Yeah. I know a few take issue with that. MedeaLysistrata posted... </cite> <quote>Fave JRPG?</quote>Great question! God, that's tough. I've played only a few but loved them all. Octopath was really fun and sticks out to me. Valkyria Chronicles too. Lost_All_Senses posted... </cite> <quote>Why don't you look at me the way you use to?</quote>I feel like all my CEmmunity building attempts has just not worked. Just feels like I'm chasing a lost thing that'll never return. <cite>[LFAQs-redacted-quote]</cite> Super Mario Odyssey, Breath of the Wild, and Super Mario Party. Been speedrunning SMO for a while, it's my favorite game ever, but I recently learned the first half of BotW any%, which has been fun. AbraCadaver posted... </cite> <quote>What is the air speed velocity of an unladen swallow?</quote>Will this be on the test? </quote> ... Copied to Clipboard!
Veggeta_EDGE 03/27/20 1:43:38 PM #8:	Were you here to witness the banning of The Admiral? --- I'm Veggeta X's alt <cite>Veggeta_EDGE posted [p:936324163] in Ask me nea... [t:78512185]...</cite> <quote>Were you here to witness the banning of The Admiral? </quote> ... Copied to Clipboard!
PatrickMahomes 03/27/20 1:44:17 PM #9:	does refs is was a less bad sack? --- NFLB 2020 Summersim Roster (6-1): https://imgur.com/4Pvr5rT St. Louis Battlehawks (3-2) <cite>PatrickMahomes posted [p:936324208] in Ask me nea... [t:78512185]...</cite> <quote>does refs is was a less bad sack? </quote> ... Copied to Clipboard!
Lost_All_Senses 03/27/20 1:46:21 PM #11:	PiOverlord posted... I feel like all my CEmmunity building attempts has just not worked. Just feels like I'm chasing a lost thing that'll never return. I feel you. That's why I out no real emotion or effort into this place. Never have the urge to actually talk about my personal life. --- Name checks out "Stupidity is a spectrum, and we're all on it" - OfDustandBone <cite>Lost_All_Senses posted [p:936324312] in Ask me nea... [t:78512185]...</cite> <quote>PiOverlord posted... </cite> <quote>I feel like all my CEmmunity building attempts has just not worked. Just feels like I'm chasing a lost thing that'll never return.</quote> I feel you. That's why I out no real emotion or effort into this place. Never have the urge to actually talk about my personal life. </quote> ... Copied to Clipboard!
Feetish 03/27/20 1:46:59 PM #12:	What is your favourite --- Thots and players <cite>Feetish posted [p:936324351] in Ask me nea... [t:78512185]...</cite> <quote>What is your favourite </quote> ... Copied to Clipboard!
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PiOverlord 03/27/20 1:58:15 PM #14:	Veggeta_EDGE posted... Were you here to witness the banning of The Admiral? I was, it was crazy. If he could get banned, any of us could. PatrickMahomes posted... does refs is was a less bad sack? Lions challenge. Lost_All_Senses posted... I feel you. That's why I out no real emotion or effort into this place. Never have the urge to actually talk about my personal life. Yeah, I have just been a bystander since my best CEer contest. Feels like I'm the least active I ever been. Feetish posted... What is your favourite Pi. --- Number of legendary 500 post topics: 33, 500th posts: 28; PiO ATTN: 5 RotM wins 1, LETTEN MY ARROW FLYEN TRUE <cite>PiOverlord posted [p:936324941] in Ask me nea... [t:78512185]...</cite> <quote>Veggeta_EDGE posted... </cite> <quote>Were you here to witness the banning of The Admiral?</quote>I was, it was crazy. If he could get banned, any of us could. PatrickMahomes posted... </cite> <quote>does refs is was a less bad sack?</quote>Lions challenge. Lost_All_Senses posted... </cite> <quote>I feel you. That's why I out no real emotion or effort into this place. Never have the urge to actually talk about my personal life.</quote>Yeah, I have just been a bystander since my best CEer contest. Feels like I'm the least active I ever been. Feetish posted... </cite> <quote>What is your favourite</quote>Pi. </quote> ... Copied to Clipboard!
Feetish 03/27/20 2:00:32 PM #15:	PiOverlord posted... Pi. Hot --- Thots and players <cite>Feetish posted [p:936325076] in Ask me nea... [t:78512185]...</cite> <quote>PiOverlord posted... </cite> <quote>Pi.</quote> Hot </quote> ... Copied to Clipboard!
PiOverlord 03/27/20 2:00:42 PM #16:	Zurkon posted... Nice, I never got around to playing Mario Odyssey but I remember everyone loving it when it came out. I want to play it at some point but there's just so many games and so little time ya know Also, I never knew you were into speed running. That's pretty cool. Is SMO the one you're best at? I have a 1:10:49. I'm probably better than most, but still far from the best. Hundreds have better times. I have WR in Super Tofu Boy, so I guess that's my best technically. --- Number of legendary 500 post topics: 33, 500th posts: 28; PiO ATTN: 5 RotM wins 1, LETTEN MY ARROW FLYEN TRUE <cite>PiOverlord posted [p:936325084] in Ask me nea... [t:78512185]...</cite> <quote>Zurkon posted... </cite> <quote>Nice, I never got around to playing Mario Odyssey but I remember everyone loving it when it came out. I want to play it at some point but there's just so many games and so little time ya know Also, I never knew you were into speed running. That's pretty cool. Is SMO the one you're best at?</quote>I have a 1:10:49. I'm probably better than most, but still far from the best. Hundreds have better times. I have WR in Super Tofu Boy, so I guess that's my best technically. </quote> ... Copied to Clipboard!
monkmith 03/27/20 2:01:33 PM #17:	A protein collected through affinity chromatography displays no activity even though it is found to have a high concentration using the Bradford protein assay. What best explains these findings? A. The Bradford reagent was prepared incorrectly. B. The active site is occupied by free ligand. C. The protein is bound to the column. D. The protein does not catalyze the reaction of interest. --- Taarsidath-an halsaam. Quando il gioco e finito, il re e il pedone vanno nella stessa scatola <cite>monkmith posted [p:936325126] in Ask me nea... [t:78512185]...</cite> <quote> A protein collected through affinity chromatography displays no activity even though it is found to have a high concentration using the Bradford protein assay. What best explains these findings? A. The Bradford reagent was prepared incorrectly. B. The active site is occupied by free ligand. C. The protein is bound to the column. D. The protein does not catalyze the reaction of interest. </quote> ... Copied to Clipboard!
PiOverlord 03/27/20 2:05:26 PM #18:	monkmith posted... A protein collected through affinity chromatography displays no activity even though it is found to have a high concentration using the Bradford protein assay. What best explains these findings? A. The Bradford reagent was prepared incorrectly. B. The active site is occupied by free ligand. C. The protein is bound to the column. D. The protein does not catalyze the reaction of interest. B. Protein activity and concentration are generally correlated. Because we have a high concentration of protein, we expect a high activity unless the protein has been damaged or inactivated in some way. The protein could have been inactivated by experimental conditions like detergents, heat, or pH; however, these are not answer choices. Rather, we must consider how the experimental procedure works. Protein elutes off of an affinity column by binding free ligand. In this situation, the binding may not have been reversed and thus the free ligand competes for the active site of the enzyme, lowering its activity. --- Number of legendary 500 post topics: 33, 500th posts: 28; PiO ATTN: 5 RotM wins 1, LETTEN MY ARROW FLYEN TRUE <cite>PiOverlord posted [p:936325339] in Ask me nea... [t:78512185]...</cite> <quote>monkmith posted... </cite> <quote>A protein collected through affinity chromatography displays no activity even though it is found to have a high concentration using the Bradford protein assay. What best explains these findings? A. The Bradford reagent was prepared incorrectly. B. The active site is occupied by free ligand. C. The protein is bound to the column. D. The protein does not catalyze the reaction of interest.</quote>B. Protein activity and concentration are generally correlated. Because we have a high concentration of protein, we expect a high activity unless the protein has been damaged or inactivated in some way. The protein could have been inactivated by experimental conditions like detergents, heat, or pH; however, these are not answer choices. Rather, we must consider how the experimental procedure works. Protein elutes off of an affinity column by binding free ligand. In this situation, the binding may not have been reversed and thus the free ligand competes for the active site of the enzyme, lowering its activity. </quote> ... Copied to Clipboard!
monkmith 03/27/20 2:08:54 PM #19:	PiOverlord posted... B. Protein activity and concentration are generally correlated. Because we have a high concentration of protein, we expect a high activity unless the protein has been damaged or inactivated in some way. The protein could have been inactivated by experimental conditions like detergents, heat, or pH; however, these are not answer choices. Rather, we must consider how the experimental procedure works. Protein elutes off of an affinity column by binding free ligand. In this situation, the binding may not have been reversed and thus the free ligand competes for the active site of the enzyme, lowering its activity. ...ok i'm gonna pull out my biochem textbook and get back to you with a question not from google... --- Taarsidath-an halsaam. Quando il gioco e finito, il re e il pedone vanno nella stessa scatola <cite>monkmith posted [p:936325534] in Ask me nea... [t:78512185]...</cite> <quote>PiOverlord posted... </cite> <quote>B. Protein activity and concentration are generally correlated. Because we have a high concentration of protein, we expect a high activity unless the protein has been damaged or inactivated in some way. The protein could have been inactivated by experimental conditions like detergents, heat, or pH; however, these are not answer choices. Rather, we must consider how the experimental procedure works. Protein elutes off of an affinity column by binding free ligand. In this situation, the binding may not have been reversed and thus the free ligand competes for the active site of the enzyme, lowering its activity.</quote>...ok i'm gonna pull out my biochem textbook and get back to you with a question not from google... </quote> ... Copied to Clipboard!
Smackems 03/27/20 2:10:28 PM #20:	Do you believe that there may be a planet devouring, bearded space worm headed right for us? Also hes a wizard --- Common sense is the most uncommon thing in the world-some dude. <cite>Smackems posted [p:936325612] in Ask me nea... [t:78512185]...</cite> <quote>Do you believe that there may be a planet devouring, bearded space worm headed right for us? Also hes a wizard </quote> ... Copied to Clipboard!
PiOverlord 03/27/20 2:10:44 PM #21:	monkmith posted... ...ok i'm gonna pull out my biochem textbook and get back to you with a question not from google... What?!? I typed that all by myself. --- Number of legendary 500 post topics: 33, 500th posts: 28; PiO ATTN: 5 RotM wins 1, LETTEN MY ARROW FLYEN TRUE <cite>PiOverlord posted [p:936325623] in Ask me nea... [t:78512185]...</cite> <quote>monkmith posted... </cite> <quote>...ok i'm gonna pull out my biochem textbook and get back to you with a question not from google...</quote>What?!? I typed that all by myself. </quote> ... Copied to Clipboard!
PiOverlord 03/27/20 2:13:30 PM #22:	Smackems posted... Do you believe that there may be a planet devouring, bearded space worm headed right for us? Also hes a wizard Yes ~~I am that worm~~ --- Number of legendary 500 post topics: 33, 500th posts: 28; PiO ATTN: 5 RotM wins 1, LETTEN MY ARROW FLYEN TRUE <cite>PiOverlord posted [p:936325759] in Ask me nea... [t:78512185]...</cite> <quote>Smackems posted... </cite> <quote>Do you believe that there may be a planet devouring, bearded space worm headed right for us? Also hes a wizard</quote>Yes I am that worm </quote> ... Copied to Clipboard!
R1masher 03/27/20 2:17:18 PM #23:	Why arent you following me? --- R1R1R1R1R1R1 <cite>R1masher posted [p:936325945] in Ask me nea... [t:78512185]...</cite> <quote>Why arent you following me? </quote> ... Copied to Clipboard!
PiOverlord 03/27/20 2:20:01 PM #24:	R1masher posted... Why arent you following me? are you sure about that? --- Number of legendary 500 post topics: 33, 500th posts: 28; PiO ATTN: 5 RotM wins 1, LETTEN MY ARROW FLYEN TRUE <cite>PiOverlord posted [p:936326089] in Ask me nea... [t:78512185]...</cite> <quote>R1masher posted... </cite> <quote>Why arent you following me?</quote>are you sure about that? </quote> ... Copied to Clipboard!
R1masher 03/27/20 2:21:04 PM #25:	Whos doing the amna now? --- R1R1R1R1R1R1 <cite>R1masher posted [p:936326153] in Ask me nea... [t:78512185]...</cite> <quote>Whos doing the amna now? </quote> ... Copied to Clipboard!
Veggeta_EDGE 03/27/20 2:42:04 PM #26:	Were you here to witness the closing of the Warned General topic series? --- I'm Veggeta X's alt <cite>Veggeta_EDGE posted [p:936327170] in Ask me nea... [t:78512185]...</cite> <quote>Were you here to witness the closing of the Warned General topic series? </quote> ... Copied to Clipboard!
PiOverlord 03/27/20 3:59:43 PM #27:	Veggeta_EDGE posted... Were you here to witness the closing of the Warned General topic series? Honestly, good riddance. I feel those kind of topics create a toxic culture. --- Number of legendary 500 post topics: 33, 500th posts: 28; PiO ATTN: 5 RotM wins 1, LETTEN MY ARROW FLYEN TRUE <cite>PiOverlord posted [p:936330995] in Ask me nea... [t:78512185]...</cite> <quote>Veggeta_EDGE posted... </cite> <quote>Were you here to witness the closing of the Warned General topic series?</quote>Honestly, good riddance. I feel those kind of topics create a toxic culture. </quote> ... Copied to Clipboard!
PatrickMahomes 03/27/20 5:47:23 PM #28:	PiOverlord posted... Honestly, good riddance. I feel those kind of topics create a toxic culture. https://i.imgur.com/dJqVZ1b.mp4 --- NFLB 2020 Summersim Roster (6-1): https://imgur.com/4Pvr5rT St. Louis Battlehawks (3-2) <cite>PatrickMahomes posted [p:936336497] in Ask me nea... [t:78512185]...</cite> <quote>PiOverlord posted... </cite> <quote>Honestly, good riddance. I feel those kind of topics create a toxic culture.</quote>https://i.imgur.com/dJqVZ1b.mp4 </quote> ... Copied to Clipboard!
AlmightyCheeks 03/27/20 5:50:41 PM #29:	Do the Martians have both sexes, like we do? --- Gamertag: ALMIGHTY CHEEKS <cite>AlmightyCheeks posted [p:936336658] in Ask me nea... [t:78512185]...</cite> <quote>Do the Martians have both sexes, like we do? </quote> ... Copied to Clipboard!
Gauss 03/27/20 5:51:19 PM #30:	Yo what it do, fam? <cite>Gauss posted [p:936336689] in Ask me nea... [t:78512185]...</cite> <quote>Yo what it do, fam? </quote> ... Copied to Clipboard!
PiOverlord 03/27/20 6:18:35 PM #31:	AlmightyCheeks posted... Do the Martians have both sexes, like we do? They are all hot alien women. Gauss posted... Yo what it do, fam? Just been speedrunning lately. Got some homework to do, but there isn't much productivity coming from me. --- Number of legendary 500 post topics: 33, 500th posts: 28; PiO ATTN: 5 RotM wins 1, LETTEN MY ARROW FLYEN TRUE <cite>PiOverlord posted [p:936338164] in Ask me nea... [t:78512185]...</cite> <quote>AlmightyCheeks posted... </cite> <quote>Do the Martians have both sexes, like we do?</quote>They are all hot alien women. Gauss posted... </cite> <quote>Yo what it do, fam?</quote>Just been speedrunning lately. Got some homework to do, but there isn't much productivity coming from me. </quote> ... Copied to Clipboard!
AbraCadaver 03/27/20 6:28:23 PM #32:	undefined posted... What is the air speed velocity of an unladen swallow? Will this be on the test? Yeah 'fraid so and you already failed, sorry man :( --- Yo ho ho and a bottle of...dumb. <cite>AbraCadaver posted [p:936338721] in Ask me nea... [t:78512185]...</cite> <quote>undefined posted... </cite> <quote>What is the air speed velocity of an unladen swallow? Will this be on the test?</quote> Yeah 'fraid so and you already failed, sorry man :( </quote> ... Copied to Clipboard!
PiOverlord 03/27/20 6:29:45 PM #33:	Dang. I'm dropping this class. Gotta only keep what makes me look good. B) --- Number of legendary 500 post topics: 33, 500th posts: 28; PiO ATTN: 5 RotM wins 1, LETTEN MY ARROW FLYEN TRUE <cite>PiOverlord posted [p:936338805] in Ask me nea... [t:78512185]...</cite> <quote>Dang. I'm dropping this class. Gotta only keep what makes me look good. B) </quote> ... Copied to Clipboard!
YookaLaylee 03/27/20 6:33:05 PM #34:	What's your favorite zombie movie? --- I'm the fly in the ointment, the spanner in the works. I'm unpredictable. I'm the X-Factor. - Jamie Madrox <cite>YookaLaylee posted [p:936339005] in Ask me nea... [t:78512185]...</cite> <quote>What's your favorite zombie movie? </quote> ... Copied to Clipboard!
PiOverlord 03/27/20 6:36:00 PM #35:	YookaLaylee posted... What's your favorite zombie movie? I don't really watch zombie movies. I think I would have to say Shaun of the Dead just because I think I maybe only saw that, WWZ, and that zombie romance movie, forget the name. --- Number of legendary 500 post topics: 33, 500th posts: 28; PiO ATTN: 5 RotM wins 1, LETTEN MY ARROW FLYEN TRUE <cite>PiOverlord posted [p:936339186] in Ask me nea... [t:78512185]...</cite> <quote>YookaLaylee posted... </cite> <quote>What's your favorite zombie movie?</quote>I don't really watch zombie movies. I think I would have to say Shaun of the Dead just because I think I maybe only saw that, WWZ, and that zombie romance movie, forget the name. </quote> ... Copied to Clipboard!
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Current Events > Ask me nearly anything (+) Yeah! (+)

Current Events > Ask me nearly anything