I honestly don't know what I would change. Anything beyond, say, the top...20 is probably pretty fluid and can change depending on how recently I've played the game and various other factors.
Just off the top of my head, I'd probably move Barret up a lot higher. He was probably the hardest character to place when I made my list the first time. I changed his spot at least 3-4 times while I was doing that list.
--
http://img.imgcake.com/leonpngpe.png
http://img.imgcake.com/squalloweenpngem.png