Not if they made it all fictional characters.
Though if they still had to have appeared in a game, I think Pinkie Pie's the only one who qualifies (even if her game was a piece of crap).
http://www.joystiq.com/2012/06/22/gameloft-bringing-my-little-pony-game-to-mobile-platforms-this-y/
Actually, these mobile games could make the Mane 6 eligible if we do go down the Fictional Character Route.
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Agent Triple Zero at your service!
The artist formerly known as greatone10.