Update:
Agent Smith (The Matrix)
Alice Twilight (No More Heroes)
Altaïr Ibn-La'Ahad (Assassin's Creed)
Anna Williams (Tekken)
Anton Chigurgh (No Country for Old Men)
Bane (DC Comics)
Beatrix Kiddo/The Bride (Kill Bill)
Boba Fett (Star Wars)
Bullseye (Marvel comics)
Cammy (Street Fighter)
Combustion Man (Avatar)
Deadpool (Marvel comics)
Desmond Miles (Assassin's Creed)
Dexter Morgan (Dexter)
Elektra (Marvel Comics)
HK-47 (Star Wars: Knights of the Old Republic)
IG-88B (Star Wars)
Jaffar (Fire Emblem 7)
James Bond (James Bond series)
Jane Smith (Mr. and Mrs. Smith)
Jason Bourne (Bourne series)
Jeego (Ghost Trick)
John Smith (Mr. and Mrs. Smith)
Legault (Fire Emblem 7)
Lettie (Final Fantasy Tactics)
Lyon (Suikoden V)
Mercenary Tao (Dragonball)
Nikita (La Femme Nikita)
O-Ren Ishii (Kill Bill)
Puss in Boots (Shrek)
Psylocke (Marvel Comics)
Rob Lucci (One Piece)
Sagiri (Suikoden V)
Sayid Jarrah (Lost)
Scar (Full Metal Alchemist)
Scorpion (Mortal Kombat)
Shadow (Final Fantasy VI)
Shigure (Suikoden V)
Shinobu (No More Heroes)
Stan Smith (American Dad!)
Starkiller (Star Wars: The Force Unleashed)
Sub-Zero (Mortal Kombat)
Taskmaster (Marvel comics)
Terminator (Terminator)
Travis Touchdown (No More Heroes)
Ulquiorra Cifer (Bleach)
Vega (Street Fighter)
Voldo (Soul series)
--
Still smilin'
:>