Update:
Ada Wong (Resident Evil)
Agent Smith (The Matrix)
Altaïr Ibn-La'Ahad (Assassin's Creed)
Assassin (Fate/Stay Night)
Bad Girl (No More Heroes)
Bane (DC Comics)
Boba Fett (Star Wars)
Bullseye (Marvel comics)
Darth Maul (Star Wars)
Desmond Miles (Assassin's Creed)
Elektra (Marvel Comics)
Elle Driver (Kill Bill)
Flonne (Disgaea)
Gray Fox (Metal Gear)
HK-47 (Star Wars: Knights of the Old Republic)
IG-88B (Star Wars)
Jason Bourne (Bourne series)
Jeego (Ghost Trick)
John Smith (Mr. and Mrs. Smith)
Kaku (One Piece)
Kitana (Mortal Kombat)
Letz Shake (No More Heroes)
Lyon (Suikoden V)
Mercenary Tao (Dragonball)
Nikita (La Femme Nikita)
Nina Williams (Tekken)
O-Ren Ishii (Kill Bill)
Puss in Boots (Shrek)
Psylocke (Marvel Comics)
Rayne (BloodRayne)
Rob Lucci (One Piece)
Sagiri (Suikoden V)
Sara (Samurai Champloo)
Scar (Full Metal Alchemist)
Scorpion (Mortal Kombat)
Shadow (Final Fantasy VI)
Shigure (Suikoden V)
Shinobu (No More Heroes)
Stan Smith (American Dad!)
Starkiller (Star Wars: The Force Unleashed)
Sub-Zero (Mortal Kombat)
T-1000 (Terminator 2)
Taskmaster (Marvel comics)
Tengo (Ghost Trick)
Terminator (Terminator)
Thane Krios (Mass Effect)
Tommy Vercetti (Grand Theft Auto: Vice City)
Travis Touchdown (No More Heroes)
Vega (Street Fighter)
Vicious (Cowboy Bebop)
Voldo (Soul series)
Volke (Fire Emblem 9/10)
Wolverine (Marvel comics)
Zeratul (Starcraft)
--
Still smilin'
:>