Note: I have not verified these assassins yet and I will only do so once the list reaches 100. I will post characters with nominations following this post:
In contest: (66/100)
Ada Wong (Resident Evil)
Agent 47 (Hitman)
Alice Twilight (No More Heroes)
Altaïr Ibn-La'Ahad (Assassin's Creed)
Anton Chigurgh (No Country for Old Men)
Assassin (Fate/Stay Night)
Beatrix Kiddo/The Bride (Kill Bill)
Boba Fett (Star Wars)
Bullseye (Marvel comics)
Cammy (Street Fighter)
Celia (Final Fantasy Tactics)
Combustion Man (Avatar)
Darth Maul (Star Wars)
Deadpool (Marvel comics)
Deadshot (DC Comics)
Dexter Morgan (Dexter)
Elektra (Marvel Comics)
Elle Driver (Kill Bill)
Ezio Auditore da Firenze (Assassin's Creed)
Flonne (Disgaea)
Garcian Smith (Killer7)
GoGo Yubari (Kill Bill)
Golgo 13 (Golgo 13)
Gray Fox (Metal Gear)
Himura Kenshin (Rurouni Kenshin)
HK-47 (Star Wars: Knights of the Old Republic)
IG-88B (Star Wars)
Jaffar (Fire Emblem 7)
James Bond (James Bond series)
Jason Bourne (Bourne series)
Jeego (Ghost Trick)
Kitana (Mortal Kombat)
Legault (Fire Emblem 7)
Leon (Leon The Professional)
Light Yagami (Death Note)
Lloyd Reed (Fire Emblem 7)
Marluxia (Kingdom Hearts)
Mileena (Mortal Kombat)
Nikita (La Femme Nikita)
Nina Williams (Tekken)
O-Ren Ishii (Kill Bill)
Sagiri (Suikoden V)
Sara (Samurai Champloo)
Sayid Jarrah (Lost)
Seta Soujiro (Rurouni Kenshin)
Shadow (Final Fantasy VI)
Shelly de Killer (Phoenix Wright series)
Shigure (Suikoden V)
Shinobu (No More Heroes)
Spy, Thy (Team Fortress 2)
Stan Smith (American Dad!)
Sub-Zero (Mortal Kombat)
T-1000 (Terminator 2)
Tengo (Ghost Trick)
Terminator (Terminator)
Thane Krios (Mass Effect)
Tommy Vercetti (Grand Theft Auto: Vice City)
Tira (Soul Calibur)
Travis Touchdown (No More Heroes)
Twilight Assassin (The Legend of Zelda)
Vega (Street Fighter)
Vegeta (Dragon Ball Z)
Vernita Green (Kill Bill)
Vicious (Cowboy Bebop)
Voldo (Soul series)
Zeratul (Starcraft)
--
Still smilin'
:>