I don't think Sephiroth should count because lol killing sitting duck Aeris.
Nor should Colm (FE8), I mean he can become an Assassin as a class, but he's essentially a small village thief IIRC.
Sub-Zero (Mortal Kombat)
Scorpion (Mortal Kombat)
Nikita (La Femme Nikita)
Vega (Street Fighter)
Anna Williams (Tekken)
Nina Williams (Tekken)
GoGo Yubari (Kill Bill)
IG-88 (Star Wars)
Light Yagami (Death Note)
Legault (Fire Emblem 7)
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SuperNiceDog won the Guru contest super nicely!