Has an anime ever been more defined by a single piece of music than this?

Roundabout for Jojo's Bizarre Adventure. That "To Be continued" with the music starting became such a huge meme

https://youtu.be/bLGHyr4U11c?si=pN75bnZHp1LOI8_R

MC_BatCommander posted...

I'll raise you FIGHT THE POWAH from Gurren Lagann, was a huge meme in its time

https://youtu.be/7W_Ja7SQD60?si=6K0xT2JEUmCNmPF_

Yes! This was another huge one. And it did actually play in scenes besides the ending, so it probably counts more for TC than my example